40S ribosome profiling reveals distinct roles for Tma20/Tma22 (MCT-1/DENR) and Tma64 (eIF2D) in 40S subunit recycling
نویسندگان
چکیده
منابع مشابه
Heterogeneity in the pre-40S ribosomal subunit reveals two distinct regions of
Ribosomes represent a significant portion of the dry mass of all cells and actively dividing cells assemble about 2,000 ribosomes every minute [1]. This represents a massive investment by the cell in a complex RNA:protein assembly that will go on to translate mRNAs into protein, an essential process for all of life. Much is known about how a ribosome faithfully performs protein synthesis; less ...
متن کاملCK1δ and CK1ε are components of human 40S subunit precursors required for cytoplasmic 40S maturation.
Biogenesis of 40S pre-ribosomal subunits requires many trans-acting factors, among them several protein kinases. In this study, we show that the human casein kinase 1 (CK1) isoforms δ and ε are required for cytoplasmic maturation steps of 40S subunit precursors. We show that both CK1δ and CK1ε isoforms are components of pre-40S subunits, on which they phosphorylate the ribosome biogenesis facto...
متن کاملRoles of the HEAT repeat proteins Utp10 and Utp20 in 40S ribosome maturation.
A family of HEAT-repeat containing ribosome synthesis factors was previously identified in Saccharomyces cerevisiae. We report the detailed characterization of two of these factors, Utp10 and Utp20, which were initially identified as components of the small subunit processome. Coprecipitation analyses confirmed the association of Utp10 and Utp20 with U3 snoRNA and the early pre-rRNA processing ...
متن کاملCoupling 40S ribosome recruitment to modification of a cap-binding initiation factor by eIF3 subunit e.
40S ribosomes are loaded onto capped mRNAs via the multisubunit translation initiation factors eIF3 and eIF4F. While eIF4E is the eIF4F cap recognition component, the eIF4G subunit associates with 40S-bound eIF3. How this intricate process is coordinated remains poorly understood. Here, we identify an eIF3 subunit that regulates eIF4F modification and show that eIF3e is required for inducible e...
متن کاملDistinct cytoplasmic maturation steps of 40S ribosomal subunit precursors require hRio2
During their biogenesis, 40S ribosomal subunit precursors are exported from the nucleus to the cytoplasm, where final maturation occurs. In this study, we show that the protein kinase human Rio2 (hRio2) is part of a late 40S preribosomal particle in human cells. Using a novel 40S biogenesis and export assay, we analyzed the contribution of hRio2 to late 40S maturation. Although hRio2 is not abs...
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ژورنال
عنوان ژورنال: Nature Communications
سال: 2021
ISSN: 2041-1723
DOI: 10.1038/s41467-021-23223-8